Physiology and Biophysics : Graduate Faculty

Ana Diez-Sampedro, Ph.D.

Assistant Professor

305-243-6670 (office)

305-243-5931 (fax)

Rosenstiel Medical Science Building - 4054

ADiezsampedro@med.miami.edu

Curriculum Vitae

1993 B.S. in Biology, University of Navarra, Spain

1998 PhD Physiology and Nutrition, University of Navarra, Spain

1999 Postdoctoral Fellowship, Department of Medicine, University of Navarra, Spain

2000-2003 Postdoctoral Fellowship, Department of Physiology, UCLA

2004-2005 Postdoctoral Fellowship, Dept. of Neurology, Yale University

2005-2006 Research Assistant Professor, Dept. of Neurology, Yale University

2007-present Assistant Professor, Dept. of Physiology and Biophysics, University of Miami School of Medicine

Research Interests

My laboratory is interested in the function of membrane proteins. In particular, our researched is focused on the activity of glucose transporters and ion channels and how the function of these proteins impact human physiology.

Recent studies include cloning and characterization of mouse SGLT1. This protein is a Na+/glucose cotransporter expressed in intestine and kidney among other tissues. In recent work, we identified an amino acid in the protein important in determining the

voltage dependence, with subsequent effects on the affinity for both Na+ and glucose.

Winners of the 2009 Stanley J. Glaser

Foundation Awards

Yanbin Zhang, PhD

Antonio Barrientos, PhD

Ana Diez-Sampedro, PhD

Zhibin Chen, MD, PhD

Hermes Jose Florez, MD, PhD

Recent Publications

Díez-Sampedro, A. Involvement of amino acid 36 in TM1 in voltage sensitivity in mouse Na+/glucose cotransporter SGLT1. J. Membr. Biol. 227(2):57-66. 2009

Wu, Y, Wang, W, Díez-Sampedro, A, Richerson, GB. . GAT-1 reverses easily and rapidly under physiological conditions. Neuron, 56:851-865. 2007

Hirayama, BA, Loo, DDF, Díez-Sampedro, A, Leung, D, Meinild, AK, Lai-Bing, M, Turk, E, Wright, EM. . Na-dependent reorganization of the sugar-binding site of SGLT1. Biochemistry, 46: 13391-13406. 2007

Voss, A.A., Díez-Sampedro, A., Hirayama, B.A., Loo, D.D.L. and Wright, E.M. Imino sugars are potent agonists of the human glucose sensor SGLT3. Mol. Pharmacol.; 71:628-34. 2007.

Díez-Sampedro A., Silverman W.R., Bautista J.F. and Richerson G.B. Mechanism of increased open probability by a mutation of the BK channel. J. Neurophysiology 96: 1507-1516. 2006.

Du, W., Bautista, J.F., Yang, H., Díez-Sampedro, A., You, S., Wang, L., Kotagal, P., Lüders, H. O., Shi, J., Cui, J., Richerson G. B. & Wang, Q. Calcium-sensitive potassium channelopathy in human epilepsy and paroxysmal movement disorder. Nature Genetics 37: 733-738. 2005.

Richerson G.B., Wang W., Hodges M.R., Dohle C.I., Díez-Sampedro A. Homing in on the specific phenotype(s) of central respiratory chemoreceptors. Exp. Physiol. 90.3: 259-269. 2005.

Díez-Sampedro, A., Loo, D.D., Wright, E.M., Zampighi, G.A., Hirayama, B.A. Coupled sodium/glucose cotransport by SGLT1 requires a negative charge at position 454.

Biochemistry 19: 13175-84. 2004.

Díez-Sampedro, A., Hirayama, B.A., Osswald, C., Gorboulev, V., Baumgarten, K., Volk, C., Wright, E.M. and Koepsell, H. A sugar sensor hiding in a family of transporters. P.N.A.S. 100: 11753-11758. 2003.

Díez-Sampedro, A., Eskandari, S., Wright, E.M. and Hirayama, B.A. Na(+)-to-sugar stoichiometry of SGLT3. Am. J. Physiol. Renal. Physiol. 280:F278-F282. 2001.

Díez-Sampedro, A., Wright, E.M. and Hirayama, B.A. Residue 457 controls sugar binding and transport in the Na+/glucose cotransporter. J. Biol. Chem. 276: 49188-49194. 2001.

Hirayama, B.A.*, Díez-Sampedro, A.* and Wright, E.M. Common mechanisms of inhibition for the Na+/glucose (hSGLT1) and Na+/Cl-/GABA (hGAT1) cotransporters. Br. J. Pharmacol. 134:484-95. 2001. * co-first author

Díez-Sampedro, A. Lostao, M. P. and Barber, A. Effects of cytochalasin E on digestive-absorptive functions. J. Physiol. Biochem. 56: 25-32. 2000.

Díez-Sampedro, A., Lostao, M.P. Wright, E.M. and Hirayama, B.A. Glycoside binding and translocation in Na+-dependent glucose cotransporters: comparison of SGLT1 and SGLT3. J. Membr. Biol. 176:111-7. 2000.

Díez-Sampedro, A., Urdaneta, E., Lostao, M.P. and Barber, A. Galactose transport inhibition by cytochalasin E. Can. J. Physiol. Pharmacol. 77:96-101. 1999.

Díez-Sampedro, A., Pérez, M., Cobo, M.T., Martínez, J.A. and Barber, A. Effect of different badrenergic agonists on the intestinal absorption of galactose and phenylalanine. J. Pharm. Pharmacol. 50:907-911. 1998.

Díez-Sampedro, A., Milagro, F.I., Berraondo, B., Zulet, M.A., Martínez, J.A. and Barber, A. Effects of Trecadrine, a b3-adrenergic agonist, on intestinal absorption of D-galactose and disaccharidase activities in three physiopathological models. J. Pharm. Pharmacol. 49: 873-877. 1997.

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