IMAGING CORE FACILITY
The Imaging Core supports the care and use of instrumentation for imaging of live and preserved organs, cells, tissues, as well as plates and arrays. The Core also provides computer software for the manipulation of images and quantification of image features.
PHOSPHOIMAGER CORE FACILITY
Mary Lou King, Ph.D.
Rosenstiel Medical Sciences Building; Room 4008
1600 NW, 10th Ave. Miami, Fl.
DESCRIPTION OF FACILITY:
Storage phosphor autoradiography system (Storm 840 PhosphorImager from Molecular Dynamics) for imaging and analysis of radioactive samples. The scanner accepts standard 20X25 cm format storage phosphor screens. The core facility does not have screens available for common use so you will have to bring your own. The system includes the ImageQuaNT data analysis software for use with Windows NT. The software will allow the investigator to quantitate radioactive samples in a linear fashion over five logs. Film is linear only over differences of three-fold. The phosphor system reduces exposure times to at least half the time required by standard x-ray film. Screens are sensitive to 32P,35S, and 125I radioisotopes.
Training in the proper use of the screens and the software programs are available for full data analysis.