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| |  Fulvia Verde, Ph.D. Associate Professor of Molecular & Cellular Pharmacology and Cell Biology; Dr. John T. MacDonald Foundation Center for Medical Genetics Miami Yeast Group member 305-243-3106 (office) 305-243-4555 (fax) Rosenstiel Medical Sciences Building 6130 fverde@med.miami.edu
1987-1992: Ph.D., Cell Biology Program, E.M.B.L., Hedelberg, Germany; Magna cum Laude
1992: Postdoctoral Research Fellow, European Molecular Biology Laboratory, Heidelberg
1992-1996: Postdoctoral Research Fellow, Imperial Cancer Research Fund, London
1996-2004: Research Assistant Professor, Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine
2004-present: Assistant Professor, Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine
Honors and Professional Activities
Member, American Society for Cell Biology (ASCB).
Member, American Society for Biochemistry and Molecular Biology (ASBMB) |
An alteration of normal cell morphology is closely associated with the onset of many disease states, including cancer, neuronal pathogenesis, and heart disease. Understanding the fundamental pathways that regulate cell shape and cell size can therefore identify novel therapeutic and diagnostic targets. Although substantial progress has been made in our understanding of morphology control, many of the molecular mechanisms involved in this process are still unknown.
By diverse microscopy, protein-protein interaction and genomic approaches, we have elucidated the structure of a novel, conserved biochemical pathway that has a fundamental function in cell morphogenesis. We have recently shown that the conserved NDR kinase, which has a role in the control of cell morphology and cell growth in many organisms ranging from yeast to mammals, regulates the spatial localization of the key morphology factor Cdc42 GTPase.
To address these questions, we are using innovative proteomic and genomic approaches, including DIGE (2-dimensional fluorescence difference gel elecrophoresis), SGA (synthetic genetic analysis) and SDL (synthetic dosage lethality). While we predominantly use the yeast S. pombe as a model system to dissect conserved molecular mechanisms of cell morphology control, we are also testing the implications of our findings in neuronal cells and in human cancer cells.
Graduate students are encouraged to present their work at national and international meetings, and are fully supported in the preparation of seminars, grants and publications.
Supported By:
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Das M, Drake T, Wiley DJ, Buchwald P, Vavylonis D, Verde F. Oscillatory Dynamics of Cdc42 GTPase in the Control of Polarized Growth. Science. 2012 May 17. [Epub ahead of print] PMID: 22604726
Das, M., Wiley, D.J., Xi Chen, X., Shah, K., and Verde, F. (2009) The conserved NDR kinase Orb6 controls polarized cell growth by spatial regulation of the small GTPase Cdc42. Current Biology, 19(15):1314-9.
Gouget K., Verde F. and Barrientos A.. (2008) In Vivo Labeling and Analysis of Mitochondrial Translation Products in the budding and in the fission yeasts. Methods Mol Biol., 457:113-124.
Das M., Wiley D.J., Medina S., Vincent H., Larrea M., Oriolo A. and Verde F. (2007) Regulation of cell diameter, For3p localization and cell symmetry by fission yeast Rho-GAP Rga4p. Molec Biol Cell, 18:2090-2101
Kanai M., Kume K., Miyahara K., Sakai K., Nakamura K., Leonhard K., Wiley D., Verde F., Toda T. and Hirata D. (2005) Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis. EMBO J, 24(17):3012-25.
Feierbach B., Verde F., and Chang F. (2004) Regulation of a formin complex by the microtubule plus end protein tea1p. J.Cell Biol., 165(5):697-707.
Oltra, E., Werner R., Verde F. and D’Urso G. (2004) A novel RING-finger-like protein Ini1 is required for cell cycle progression in fission yeast. J.Cell Sci 117(Pt 6):967-74.
Kim H., Yang P., Catanuto, P., Verde F., Lai H., Du H., Chang F., and Marcus, S. (2003) The kelch-repeat protein, Tea1, is a potential substrate target of the p21-activated kinase Shk1, in the fission yeast Schizosaccharomyces pombe. J. Biol. Chem. 278:30074-82.
Chang, F. and Verde, F. Control of cell polarity and cell morphogenesis. (2003) In: “Molecular Biology of Schizosaccharomyces pombe” Springer-Verlag, 255-267.
Wiley DJ, Marcus S., D’Urso G. and Verde, F. (2003) Control of cell polarity in fission yeast by association of Orb6p kinase with the highly conserved protein methyltransferase Skb1p. J. Biol. Chem. 278(27):25256-63.
Hou M., Wiley D., Verde F. and McCollum D. (2003) Mob2p interacts with Orb6p kinase to promote coordination of cell polarity with cell cycle progression. J. Cell Science, 116 (Pt. 1): 125-35.
Bao S., Qyang Y., Yang P., Kim H., Du H., Bartholomeusz G., Pimental R., Verde F., Marcus S. (2001) The highly conserved protein methyltransferase, Skb1, is a mediator of hyperosmotic stress response in fission yeast, Schizosaccharomyces pombe. J. Biol. Chem. 276 (18): 14549-52.
Verde, F., D.J. Wiley and P. Nurse, (1998) Fission yeast orb6, a ser/thr protein kinase related to mammalian rho-kinase and myotonic dystrophy kinase, interacts with pak1/shk1 and co-ordinates cell morphogenesis with the cell cycle. Proc. Natl. Acad. Sci. USA, 95, 7526-7531.
Tournebize, R., SSL Andersen , F. Verde, M. Dorée, E. Karsenti and A.A. Hyman, (1997) Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis. EMBO J 16(18), 5537-5549.
Verde, F., J. Mata and P. Nurse, (1995) Fission yeast cell morphogenesis: Identification of new genes and analysis of their role during the cell cycle. J. Cell Biol. 131(6), 1529-1538.
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